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Water quality coliform detection - enzyme substrate detection system - Database & Sql Blog Articles
The Water Quality Coliform Enzyme Substrate Detection System is a modern and efficient solution for testing water samples. It includes the LK-2010 program-controlled quantitative sealing machine, 51 or 97-well quantitative detection trays, 100mL quantitative bottles, and enzyme substrate reagents. This system can be used for various types of water samples, including drinking water, source water, bottled water, medium water, secondary water supply, pipeline water, wastewater, food water, livestock water, and medical water.
The detection method utilizes Escherichia coli to produce β-galactosidase, which breaks down the color substrate ONPG (Ortho-nitrophenyl-β-D-galactopyranoside), turning the culture solution yellow. Additionally, E. coli produces β-glucuronidase, which decomposes MUG (4-methyl-umbelliferyl-β-glucuronide) and causes fluorescence at 366 nm, allowing for the qualitative and quantitative analysis of total coliforms, fecal coliforms, and E. coli in water.
Here are the technical specifications:
| Serial Number | Indicator Name | Technical Indicators |
|---------------|------------------------|---------------------------------------------------------------------------------------|
| 1 | Name | Program-controlled quantitative sealing machine |
| 2 | Use | Used for GB5750-2006 Enzyme substrate method for detection of total coliforms, E. coli, and fecal coliforms |
| 3 | Reliability | No leakage, no holes |
| 4 | Stability | Detectable for more than 40,000 samples, service life over 5 years |
| 5 | Convenience | Open/Close, quantitative digital display window, cleaning window |
| 6 | Quickness | No need for a sterile room, 24-hour detection of total coliforms, E. coli, and heat-resistant coliforms |
| 7 | Weight | ≤16 kg |
| 8 | Size | 39 cm long × 27 cm wide × 30 cm high |
| 9 | Preheat Time | ≤14 minutes |
| 10 | Noise | <50 dBa |
| 11 | Cover Temperature | <40°C |
| 12 | Sealing Speed | - |
| 13 | Operating Voltage | AC 220V ±10%, 50Hz |
| 14 | Sealing Speed | 51-hole and 97-hole quantitative detection disk sealing time ≤12 seconds per unit |
| 15 | Working Temperature | -10°C to 50°C |
| 16 | Examination Range | 51-hole: 0–200 MPN/100ml (no dilution); 97-hole: 0–2419 MPN/100ml (no dilution) |
This detection method is widely used in developed countries around the world. Compared to traditional Chinese methods like the multiple-tube fermentation and membrane filtration techniques, it offers better selectivity, lower false positive rates, and greater accuracy. The convenience and speed of this method significantly improve laboratory efficiency and reduce testing time. In emergency monitoring scenarios, it enables timely data reporting, helping to prevent large-scale public safety incidents.
