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Water quality coliform detection - enzyme substrate detection system - Database & Sql Blog Articles
The Water Quality Coliform Enzyme Substrate Detection System is a modern and efficient solution for the rapid detection of coliform bacteria, including total coliforms, fecal coliforms, and Escherichia coli in various water samples. This system includes the LK-2010 program-controlled quantitative sealing machine, 51 or 97-well quantitative detection trays, 100mL quantitative bottles, and enzyme substrate reagents. It is suitable for testing drinking water, source water, bottled water, wastewater, food water, medical water, and more.
The detection method utilizes the enzymatic activity of E. coli to break down specific substrates. For instance, β-galactosidase from E. coli converts ONPG (ortho-nitrophenyl-β-D-galactopyranoside) into a yellow compound, while β-glucuronidase breaks down MUG (4-methylumbelliferyl-β-glucuronide), causing fluorescence at 366 nm. This allows for both qualitative and quantitative analysis of coliforms in water samples.
Key technical specifications include:
| Serial Number | Indicator Name | Technical Indicators |
|---------------|------------------------|---------------------------------------------------------------------------------------|
| 1 | Name | Program-controlled quantitative sealing machine |
| 2 | Use | Used for GB5750-2006 Enzyme substrate method for detection of total coliforms, E. coli, and fecal coliforms |
| 3 | Reliability | No leakage, no holes |
| 4 | Stability | Detectable over 40,000 samples, service life over 5 years |
| 5 | Convenience | Open/Off and backspace, digital display window, cleaning window |
| 6 | Quickness | No need for a sterile room, 24h detection for total coliforms, E. coli, and heat-resistant coliforms |
| 7 | Weight | ≤16kg |
| 8 | Size | 39cm x 27cm x 30cm |
| 9 | Preheat Time | ≤14 minutes |
| 10 | Noise | <50dba |
| 11 | Cover Temperature | <40°C |
| 12 | Sealing Speed | 51-hole and 97-hole quantitative detection disk sealing time ≤12 seconds per unit |
| 13 | Operating Voltage | AC 220V ±10%, 50Hz |
| 14 | Working Temperature | -10°C to 50°C |
| 15 | Examination Range | 51-hole: 0–200 MPN/100ml (no dilution); 97-hole: 0–2419 MPN/100ml (no dilution) |
This detection method is widely used in developed countries and offers significant advantages over traditional methods like the multi-tube fermentation and membrane filtration techniques. It provides high specificity, reduces false positives, and ensures accurate results. The system is not only fast and user-friendly but also enhances laboratory efficiency and enables timely reporting during emergency monitoring, which is crucial for preventing large-scale public health incidents.
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(1) Check the original appearance of the circuit board. Pay attention to cleanliness and appearance inspection.
(2) Inspect the mask, including specified color, specified thickness, quality defects, and pitting.
(3) Is electroplating sufficient? Adequate electroplating is difficult to measure. If the requirements are strict, the current requirement is based on the plating thickness.
(4) Is the hole centered around the pad (annular ring and alignment)?
(5) Is the hole size correct?
(6) Does the appearance match the artwork?
(7) Does the steel mesh match the printing position of the circuit board?
(8) Is the overall circuit board size correct?
(9) Is the PCB warped?
(10) Is there copper on the edge of the PCB board?
(11) PCB tracking width tolerance.